Essential genes predicted in the genome of Rubrivivax gelatinosus
In: Journal of Bacteriology, Jg. 198 (2016-08-01), Heft 15-16, S. 2244-2250
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Zugriff:
Rubrivivax gelatinosus is a betaproteobacterium with impressive metabolic diversity. It is capable of phototrophy, chemotrophy, two different mechanisms of sugar metabolism, fermentation, and H.sub.2 gas production. To identify core essential genes, R. gelatinosus was subjected to saturating transposon mutagenesis and high-throughput sequencing (TnSeq) analysis using nutrient-rich, aerobic conditions. Results revealed that virtually no primary metabolic genes are essential to the organism and that genomic redundancy only explains a portion of the nonessentiality, but some biosynthetic pathways are still essential under nutrient-rich conditions. Different essentialities of different portions of the Pho regulatory pathway suggest that overexpression of the regulon is toxic and hint at a larger connection between phosphate regulation and cellular health. Lastly, various essentialities of different tRNAs hint at a more complex situation than would be expected for such a core process. These results expand upon research regarding cross-organism gene essentiality and further enrich the study of purple nonsulfur bacteria.
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Essential genes predicted in the genome of Rubrivivax gelatinosus
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Autor/in / Beteiligte Person: | Curtis, Patrick D. |
Zeitschrift: | Journal of Bacteriology, Jg. 198 (2016-08-01), Heft 15-16, S. 2244-2250 |
Veröffentlichung: | 2016 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
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