Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function
In: Journal of Bacteriology, Jg. 196 (2014-04-01), Heft 7-8, S. 1318-1324
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Zugriff:
Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporulation, while YqjG stimulates competence development. To obtain more insight into the regions important for the sporulation specificity of SpoIIIJ, a series of SpoIIIJ/YqjG chimeras was constructed. These chimeras were tested for functionality during vegetative growth and for their ability to complement the sporulation defect of a spoIIIJ deletion strain. The data suggest an important role for the domain comprising transmembrane segment 2 (TMS2) and its flanking loops in sporulation specificity, with lesser contributions to specificity by TMS1 and TMS3.
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Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function
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Autor/in / Beteiligte Person: | Geng, Yanping ; de Keyzer, Jeanine ; Scheffers, Dirk-Jan ; Driessen, Arnold J. M. |
Zeitschrift: | Journal of Bacteriology, Jg. 196 (2014-04-01), Heft 7-8, S. 1318-1324 |
Veröffentlichung: | 2014 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
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