CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells
In: International Journal of Molecular Sciences, Jg. 24 (2023-02-01), Heft 4, S. 3399-3399
Online
academicJournal
Zugriff:
CBL is rapidly phosphorylated upon insulin receptor activation. Mice whole body CBL depletion improved insulin sensitivity and glucose clearance; however, the precise mechanisms remain unknown. We depleted either CBL or its associated protein SORBS1/CAP independently in myocytes and assessed mitochondrial function and metabolism compared to control cells. CBL- and CAP-depleted cells showed increased mitochondrial mass with greater proton leak. Mitochondrial respiratory complex I activity and assembly into respirasomes were reduced. Proteome profiling revealed alterations in proteins involved in glycolysis and fatty acid degradation. Our findings demonstrate CBL/CAP pathway couples insulin signaling to efficient mitochondrial respiratory function and metabolism in muscle.
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CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells
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Autor/in / Beteiligte Person: | Aye, Cho-Cho ; Hammond, Dean E. ; Rodriguez-Cuenca, Sergio ; Doherty, Mary K. ; Whitfield, Phillip D. ; Phelan, Marie M. ; Yang, Chenjing ; Perez-Perez, Rafael ; Li, Xiaoxin ; Diaz-Ramos, Angels ; Peddinti, Gopal ; Oresic, Matej ; Vidal-Puig, Antonio ; Zorzano, Antonio ; Ugalde, Cristina ; Mora, Silvia |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 24 (2023-02-01), Heft 4, S. 3399-3399 |
Veröffentlichung: | MDPI AG, 2023 |
Medientyp: | academicJournal |
ISSN: | 1422-0067 (print) ; 1661-6596 (print) |
DOI: | 10.3390/ijms24043399 |
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