hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility
In: Cell Reports, Jg. 43 (2024), Heft 2, S. 113769-
Online
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Zugriff:
Summary: Although the composition and assembly of stress granules (SGs) are well understood, the molecular mechanisms underlying SG disassembly remain unclear. Here, we identify that heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) is associated with SGs and that its absence specifically enhances the disassembly of arsenite-induced SGs depending on the ubiquitination-proteasome system but not the autophagy pathway. hnRNPA2B1 interacts with many core SG proteins, including G3BP1, G3BP2, USP10, and Caprin-1; USP10 can deubiquitinate G3BP1; and hnRNPA2B1 depletion attenuates the G3BP1-USP10/Caprin-1 interaction but elevates the G3BP1 ubiquitination level under arsenite treatment. Moreover, the disease-causing mutation FUSR521C also disassembles faster from SGs in HNRNPA2B1 mutant cells. Furthermore, knockout of hnRNPA2B1 in mice leads to Sertoli cell-only syndrome (SCOS), causing complete male infertility. Consistent with this, arsenite-induced SGs disassemble faster in Hnrnpa2b1 knockout (KO) mouse Sertoli cells as well. These findings reveal the essential roles of hnRNPA2B1 in regulating SG disassembly and male mouse fertility.
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hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility
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Autor/in / Beteiligte Person: | Wang, Xiaoli ; Fan, Xu ; Zhang, Jin ; Wang, Fengli ; Chen, Jingshou ; Wen, Yujiao ; Wang, Lingjuan ; Li, Tao ; Li, Huaibiao ; Gu, Heng ; Zhang, Youzhi ; Yuan, Shuiqiao |
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Zeitschrift: | Cell Reports, Jg. 43 (2024), Heft 2, S. 113769- |
Veröffentlichung: | Elsevier, 2024 |
Medientyp: | academicJournal |
ISSN: | 2211-1247 (print) |
DOI: | 10.1016/j.celrep.2024.113769 |
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