Protein Disulfide Isomerase 4 Is an Essential Regulator of Endothelial Function and Survival
In: International Journal of Molecular Sciences, Jg. 25 (2024-03-01), Heft 7, S. 3913-3913
Online
academicJournal
Zugriff:
Endothelial autophagy plays an important role in the regulation of endothelial function. The inhibition of endothelial autophagy is associated with the reduced expression of protein disulfide isomerase 4 (PDIA-4); however, its role in endothelial cells is not known. Here, we report that endothelial cell-specific loss of PDIA-4 leads to impaired autophagic flux accompanied by loss of endothelial function and apoptosis. Endothelial cell-specific loss of PDIA-4 also induced marked changes in endothelial cell architecture, accompanied by the loss of endothelial markers and the gain of mesenchymal markers consistent with endothelial-to-mesenchymal transition (EndMT). The loss of PDIA-4 activated TGFβ-signaling, and inhibition of TGFβ-signaling suppressed EndMT in PDIA-4-silenced endothelial cells in vitro. Our findings help elucidate the role of PDIA-4 in endothelial autophagy and endothelial function and provide a potential target to modulate endothelial function and/or limit autophagy and EndMT in (patho-)physiological conditions.
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Protein Disulfide Isomerase 4 Is an Essential Regulator of Endothelial Function and Survival
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Autor/in / Beteiligte Person: | Bu, Shuhan ; Singh, Aman ; Nguyen, Hien C. ; Peddi, Bharatsinai ; Bhatt, Kriti ; Ravendranathan, Naresh ; Frisbee, Jefferson C. ; Singh, Krishna K. |
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Zeitschrift: | International Journal of Molecular Sciences, Jg. 25 (2024-03-01), Heft 7, S. 3913-3913 |
Veröffentlichung: | MDPI AG, 2024 |
Medientyp: | academicJournal |
ISSN: | 1422-0067 (print) ; 1661-6596 (print) |
DOI: | 10.3390/ijms25073913 |
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