F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells
In: iScience, Jg. 26 (2023), Heft 8, S. 107384-
Online
academicJournal
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Summary: Our study focused on deciphering the role of F-actin and related regulatory factors during SARS-CoV-2 particle production and transmission in human pulmonary cells. Quantitative high-resolution microscopies revealed that the late phases of SARS-CoV-2 infection induce a strong rearrangement of F-actin nanostructures dependent on the viral M, E, and N structural proteins. Intracellular vesicles containing viral components are labeled with Rab7 and Lamp1 and are surrounded by F-actin ring-shaped structures, suggesting their role in viral trafficking toward the cell membrane for virus release. Furthermore, filopodia-like nanostructures were loaded with viruses, potentially facilitating their egress and transmission between lung cells. Gene expression analysis revealed the involvement of alpha-actinins under the regulation of the protein kinase N (PKN). The use of a PKN inhibitor efficiently reduces virus particle production, restoring endoplasmic reticulum and F-actin cellular shape. Our results highlight an important role of F-actin rearrangements during the productive phases of SARS-CoV-2 particles.
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F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells
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Autor/in / Beteiligte Person: | Swain, Jitendriya ; Merida, Peggy ; Rubio, Karla ; Bracquemond, David ; Neyret, Aymeric ; Aguilar-Ordoñez, Israel ; Günther, Stefan ; Barreto, Guillermo ; Muriaux, Delphine |
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Zeitschrift: | iScience, Jg. 26 (2023), Heft 8, S. 107384- |
Veröffentlichung: | Elsevier, 2023 |
Medientyp: | academicJournal |
ISSN: | 2589-0042 (print) ; 2663-7340 (print) |
DOI: | 10.1016/j.isci.2023.107384 |
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