Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
In: Nature Communications, Jg. 15 (2024), Heft 1, S. 1-17
Online
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Zugriff:
Abstract Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle cell (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting cells originate from vSMC precursors. These pericyte-like precursors were recently identified in the aorta-gonad-mesonephros (AGM) region; however, their role in the hematopoietic development in vivo remains unknown. Here, we identify a subpopulation of NG2+Runx1+ perivascular cells that display a sclerotome-derived vSMC transcriptomic profile. We show that deleting Runx1 in NG2+ cells impairs the hematopoietic development in vivo and causes transcriptional changes in pericytes/vSMCs, endothelial cells and hematopoietic cells in the murine AGM. Importantly, this deletion leads also to a significant reduction of HSC reconstitution potential in the bone marrow in vivo. This defect is developmental, as NG2+Runx1+ cells were not detected in the adult bone marrow, demonstrating the existence of a specialised pericyte population in the HSC-generating niche, unique to the embryo.
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Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo
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Autor/in / Beteiligte Person: | Zaniah N. Gonzalez Galofre ; Kilpatrick, Alastair M. ; Marques, Madalena ; Diana Sá da Bandeira ; Ventura, Telma ; Mario Gomez Salazar ; Bouilleau, Léa ; Marc, Yvan ; Barbosa, Ana B. ; Rossi, Fiona ; Beltran, Mariana ; Harmen J. G. van de Werken ; Wilfred F. J. van IJcken ; Henderson, Neil C. ; Forbes, Stuart J. ; Crisan, Mihaela |
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Zeitschrift: | Nature Communications, Jg. 15 (2024), Heft 1, S. 1-17 |
Veröffentlichung: | Nature Portfolio, 2024 |
Medientyp: | academicJournal |
ISSN: | 2041-1723 (print) |
DOI: | 10.1038/s41467-024-44913-z |
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