Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions
In: iScience, Jg. 27 (2024), Heft 4, S. 109411-
Online
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Summary: To investigate the impact of paracrine IL-2 signals on memory precursor (MP) cell differentiation, we activated CD8 T cell in vitro in the presence or absence of exogenous IL-2 (ex-IL-2). We assessed memory differentiation by transferring these cells into virus-infected mice. Both conditions generated CD8 T cells that participate in the ongoing response and gave rise to similar memory cells. Nevertheless, when transferred into a naive host, T cells activated with ex-IL-2 generated a higher frequency of memory cells displaying increased functional memory traits. Single-cell RNA-seq analysis indicated that without ex-IL-2, cells rapidly acquire an MP signature, while in its presence they adopted an effector signature. This was confirmed at the protein level and in a functional assay. Overall, ex-IL-2 delays the transition into MP cells, allowing the acquisition of effector functions that become imprinted in their progeny. These findings may help to optimize the generation of therapeutic T cells.
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Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions
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Autor/in / Beteiligte Person: | Wang, Shaoying ; Prieux, Margaux ; Simon de Bernard ; Dubois, Maxence ; Laubreton, Daphne ; Djebali, Sophia ; Zala, Manon ; Arpin, Christophe ; Genestier, Laurent ; Leverrier, Yann ; Gandrillon, Olivier ; Crauste, Fabien ; Jiang, Wenzheng ; Marvel, Jacqueline |
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Zeitschrift: | iScience, Jg. 27 (2024), Heft 4, S. 109411- |
Veröffentlichung: | Elsevier, 2024 |
Medientyp: | academicJournal |
ISSN: | 2589-0042 (print) |
DOI: | 10.1016/j.isci.2024.109411 |
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