Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
In: Cell Reports, Jg. 42 (2023), Heft 10, S. 113232-
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Summary: TRPM7 (transient receptor potential cation channel subfamily M member 7) is a chanzyme with channel and kinase domains essential for embryo development. Using gamete-specific Trpm7-null lines, we report that TRPM7-mediated Mg2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is expressed dynamically from gametes to blastocysts; displays stage-specific localization on the plasma membrane, cytoplasm, and nucleus; and undergoes cleavage that produces C-terminal kinase fragments. TRPM7 underpins Mg2+ homeostasis, and excess Mg2+ but not Zn2+ or Ca2+ overcomes the arrest of Trpm7-null embryos; expressing Trpm7 mRNA restores development, but mutant versions fail or are partially rescued. Transcriptomic analyses of Trpm7-null embryos reveal an abundance of oxidative stress-pathway genes, confirmed by mitochondrial dysfunction, and a reduction in transcription factor networks essential for proliferation; Mg2+ supplementation corrects these defects. Hence, TRPM7 underpins Mg2+ homeostasis in preimplantation embryos, prevents oxidative stress, and promotes gene expression patterns necessary for developmental progression and cell-lineage specification.
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Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes
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Autor/in / Beteiligte Person: | Gupta, Neha ; Soriano-Úbeda, Cristina ; Stein, Paula ; Savy, Virginia ; Papas, Brian N. ; Ardestani, Goli ; Carvacho, Ingrid ; Alfandari, Dominique ; Williams, Carmen J. ; Fissore, Rafael A. |
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Zeitschrift: | Cell Reports, Jg. 42 (2023), Heft 10, S. 113232- |
Veröffentlichung: | Elsevier, 2023 |
Medientyp: | academicJournal |
ISSN: | 2211-1247 (print) |
DOI: | 10.1016/j.celrep.2023.113232 |
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