Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
In: STAR Protocols, Jg. 3 (2022), Heft 2, S. 101324-
Online
academicJournal
Zugriff:
Summary: This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleavage, which enables detailed investigation of protein function. This experimental strategy overcomes some of the limitations associated with gene knockouts in T. cruzi.For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).
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Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
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Autor/in / Beteiligte Person: | Gisele Fernanda Assine Picchi-Constante ; Priscila Mazzocchi Hiraiwa ; Marek, Martin ; Vanessa Zulkievicz Rogerio ; Eloise Pavão Guerra-Slompo ; Romier, Christophe ; Nilson Ivo Tonin Zanchin |
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Zeitschrift: | STAR Protocols, Jg. 3 (2022), Heft 2, S. 101324- |
Veröffentlichung: | Elsevier, 2022 |
Medientyp: | academicJournal |
ISSN: | 2666-1667 (print) |
DOI: | 10.1016/j.xpro.2022.101324 |
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