Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages
In: Asian Pacific Journal of Tropical Biomedicine, Jg. 4 (2014), Heft 3, S. 219-227
Online
academicJournal
Zugriff:
Objective: To investigate antioxidant and anti-inflammatory effects of Tagetes minuta (T. minuta) essential oil. Methods: In the present study T. minuta essential oil was obtained from leaves of T. minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry. The anti-oxidant capacity of T. minuta essential oil was examined by measuring reactive oxygen, reactive nitrogen species and hydrogen peroxide scavenging. The anti-inflammatory activity of T. minuta essential oil was determined through measuring NADH oxidase, inducible nitric oxide synthase and TNF-α mRNA expression in lipopolysacharide-stimulated murine macrophages using real-time PCR. Results: Gas chromatography-mass spectrometry analysis indicated that the main components in the T. minuta essential oil were dihydrotagetone (33.86%), E-ocimene (19.92%), tagetone (16.15%), cis-β-ocimene (7.94%), Z-ocimene (5.27%), limonene (3.1%) and epoxyocimene (2.03%). The T. minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC50 12-15 μg/mL, which indicated a potent radical scavenging activity. In addition, T. minuta essential oil significantly reduced NADH oxidase, inducible nitric oxide synthaseand TNF-α mRNA expression in the cells at concentrations of 50 μg/mL, indicating a capacity of this product to potentially modulate/diminish immune responses. Conclusions: T. minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions.
Titel: |
Anti–oxidative and anti–inflammatory effects of Tagetes minuta essential oil in activated macrophages
|
---|---|
Autor/in / Beteiligte Person: | Karimian, Parastoo ; Kavoosi, Gholamreza ; Amirghofran, Zahra |
Link: | |
Zeitschrift: | Asian Pacific Journal of Tropical Biomedicine, Jg. 4 (2014), Heft 3, S. 219-227 |
Veröffentlichung: | Wolters Kluwer Medknow Publications, 2014 |
Medientyp: | academicJournal |
ISSN: | 2221-1691 (print) |
DOI: | 10.1016/S2221-1691(14)60235-5 |
Schlagwort: |
|
Sonstiges: |
|