α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells
In: International Journal of Molecular Sciences, Vol 25, Iss 2, p 1252 (2024, Jg. 25 (2024), Heft 2, p 1252
Online
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Zugriff:
Glucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for glucose uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to the cell plasma membrane is reduced, compromising cell energy metabolism. Therefore, the use of synthetic or naturally occurring molecules able to stimulate GLUT4 expression represents a good tool for alternative treatments of insulin resistance. The present study aimed to investigate the effects of essential oils (EOs) derived from Pinus spp. ( P. nigra and P. radiata ) and of their main terpenoid constituents (α- and β-pinene) on the expression/translocation of GLUT4 in myoblast C2C12 murine cells. For this purpose, the chemical profiles of the EOs were first analyzed through gas chromatography–mass spectrometry (GC-MS). Cell viability was assessed by MTT assay, and GLUT4 expression/translocation was evaluated through RT-qPCR and flow cytometry analyses. The results showed that only the P. nigra essential oil (PnEO) and α-pinene can increase the transcription of the Glut4/Scl2a4 gene, resulting in a subsequent increase in the amount of GLUT4 produced and its plasma membrane localization. Moreover, the PnEO or α-pinene can induce Glut4 expression both during myogenesis and in myotubes. In summary, the PnEO and α-pinene emulate insulin’s effect on the GLUT4 transporter expression and its translocation to the muscle cell surface.
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α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells
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Autor/in / Beteiligte Person: | Feriotto, Giordana ; Tagliati, Federico ; Costa, Valentina ; Monesi, Marcello ; Tabolacci, Claudio ; Beninati, Simone ; Mischiati, Carlo |
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Zeitschrift: | International Journal of Molecular Sciences, Vol 25, Iss 2, p 1252 (2024, Jg. 25 (2024), Heft 2, p 1252 |
Veröffentlichung: | MDPI AG, 2024 |
Medientyp: | academicJournal |
ISSN: | 1422-0067 (print) ; 1661-6596 (print) |
DOI: | 10.3390/ijms25021252 |
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