While K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensable
In: $2, 2003, S. 9245-9250
Online
academicJournal
Zugriff:
In mammals, the three classical ras genes encode four highly homologous proteins, N-Ras, H-Ras, and the isoforms K-Ras 4A and 4B. Previous studies have shown that K-ras is essential for mouse development and that while K-ras 4A and 4B are expressed during development, K-ras 4A expression is regulated temporally and spatially and occurs in adult kidney, intestine, stomach, and liver. In the present study, the pattern of K-ras 4A expression was examined in a wide range of wild-type adult mouse tissues, and gene targeting was used to generate K-ras 4A-deficient mice to examine its role in development. It was found that K-ras 4A is also expressed in uterus, lung, pancreas, salivary glands, seminal vesicles, bone marrow cells, and cecum, where it was the major K-Ras isoform expressed. Mating between K-ras(tmDelta4A/+) mice produced viable K-ras(tmDelta4A/tmDelta4A) offspring with the expected Mendelian ratios of inheritance, and these mice expressed the K-ras 4B splice variant only. K-ras(tmDelta4A/tmDelta4A) mice were fertile and showed no histopathological abnormalities on inbred (129/Ola) or crossbred (129/Ola x C57BL/6) genetic backgrounds. The results demonstrate that K-Ras 4A, like H- and N-Ras, is dispensable for normal mouse development, at least in the presence of functional K-Ras 4B.
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While K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensable
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Autor/in / Beteiligte Person: | Plowman, Sarah J ; Williamson, D James ; O'Sullivan, Maureen J ; Doig, Jennifer ; Ritchie, Ann-Marie ; Harrison, David J ; Melton, David W ; Arends, Mark J ; Hooper, Martin L ; Patek, Charles E |
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Zeitschrift: | $2, 2003, S. 9245-9250 |
Veröffentlichung: | 2003 |
Medientyp: | academicJournal |
DOI: | 10.1128/MCB.23.24.9245-9250.2003 |
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