Rbcn-3A is essential for Yl recycling and yolk granule biogenesis.
2024
Online
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Zugriff:
Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of dsRNA against Rbcn-3A, double-labeled with phalloidin (red) and antibody against (A, B) endogenous Rab7 (green) or (C, D) GFP (green) from flies (C) with ectopic expression of GFP-2xFYVE or (D) carrying a genome-tagging Yl-eGFP-3xHA. Images are presented as overlaying images in color or in individual channels in gray, as annotated. (B-B3, C1, D1) High-magnification views of the cortex regions highlighted in (A, C, D), respectively, as annotated. Genotypes: The samples were from adult females flies heterozygous for both matalpha4-GAL-VP16 driver (BDSC #7062) and the following UAS-transgenic RNAi and PI(3)P or Yl reporter lines: (A,B) P{TRiP.HMS01287}attP2 (#34612). (C) P{w(+mC) = UAS-GFP-myc-2xFYVE}2 (#42172); P{TRiP. HMS01287}attP2 (#34612). (D). P{TRiP.HMS01287}attP2 (#34612); p{mini-W+, yl -eGFP-3xHA}. The sizes of the scale bars as annotated.
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Rbcn-3A is essential for Yl recycling and yolk granule biogenesis.
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Autor/in / Beteiligte Person: | Yu, Yue ; Chen, Dongsheng ; Farmer, Stephen M. ; Xu, Shiyu ; Rios, Beatriz ; Solbach, Amanda ; Ye, Xin ; Ye, Lili ; Zhang, Sheng |
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Veröffentlichung: | 2024 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pgen.1011152.g007 |
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