Influence of Melaleuca cajuputi Powell and Cymbopogon citratus Essential Oil Formulated in Alcohol Free Mouthwash against Candida albicans Culture ; อิทธิพลของน้ำมันหอมระเหยเสม็ดขาวและตะไคร้ที่ผสมในตำรับน้ำยาบ้วนปากปราศจากแอลกอฮอล์ เพื่อต้านแคนดิดาอัลบิแคนส์

Aim: To study the influence of Melaleuca cajuputi Powell and Cymbopogon citratus essential oils formulated in alcohol free mouthwash against Candida albicans culture Material and Methods: Melaleuca cajuputi Powell or/and Cymbopogon citratus essential oils were selected to be formulated in alcohol free mouthwash. The mouthwash containing certain concentrations in the range of 0 - 250 µl/ml of each essential oil was primarily determined for inhibitory effect against C. albicans ATCC 10231 culture by disc diffusion and broth microdilution methods. The lowest concentrations of both essential oils significantly inhibiting the growth of C. albicans were formulated in alcohol free mouthwash. All three formulas of alcohol free mouthwash containing either Melaleuca cajuputi Powell or Cymbopogon citratus or both essential oils were determined for their anti-candidal activity as mentioned above. Results: By disc diffusion method, no inhibition zone against C. albicans was observed from the alcohol free mouthwash containing Melaleuca cajuputi Powell or Cymbopogon citratus with the concentrations less than 250 µl/ml or 120 µl/ml respectively. In contrast, by broth microdilution method, the mouthwash formulated with 8 µl/ml Melaleuca cajuputi Powell or 4 µl/ml Cymbopogon citratus essential oil were the lowest concentrations significantly inhibiting the growth of C. albicans by 80% approximately. Moreover, the mouthwash formulated with both Melaleuca cajuputi Powell (8 µl/ml) and Cymbopogon citratus (4 µl/ml) showed relatively stronger inhibitory effect against C. albicans (> 80%), compared to the ones formulated without or with single essential oil. Conclusions: The alcohol free mouthwash newly formulated with mixed Melaleuca cajuputi Powell and Cymbopogon citratus essential oils expressed more potent anti-candida effect than the others formulated without or with individual essential oil. ; วัตถุประสงค์: ...