Chemical composition and the insecticidal activity of Aeollanthus pubescens leaf essential oil against Anopheles gambiae sensu stricto
In: ISSN: 1756-3305, 2021
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International audience ; Background: The excessive use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties is an alternative approach to the use of synthetic insecticides. The aim of this study is to investigating the larvicidal and adulticidal activity and the chemical composition of the essential oil of Aeollanthus pubescens on the major malaria vector, Anopheles gambiae. Methods: Three reference strains of Anopheles gambiae sensu stricto (Kisumu, Kiskdr and Acerkis) were used in this study. The leaves of A. pubescens were collected in southern Benin. The standard World Health Organisation (WHO) guidelines for larvicide evaluation were used, and the chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Adult mosquitoes of each strain were exposed to pieces of net coated with the essential oil for 3 min using the WHO cone bioassay method. Probit regression analysis was used to determine the concentrations that would kill 50 and 95% of each test population (LC 50 , LC 95) and the knockdown time for 50 and 95% of each test population (KDT 50 , and KDT 95). The difference between the mortality-dose regressions for the different strains was analysed using the likelihood ratio test (LRT). The log-rank test was performed to evaluate the difference in survival between the strains. Results: A total of 14 components were identified, accounting for 98.3% of total oil content. The major components were carvacrol (51.1%), thymyle acetate (14.0%) and ɣ-terpinene (10.6%). The essential oil showed larvicidal properties on the Kisumu, Acerkis and Kiskdr strains, with LC 50 of 29.6, 22.9 and 28.4 ppm, respectively. With pieces of netting treated at 165 µg/cm 2 , the KDT 50 of both Acerkis (1.71 s; Z = 3.34, P < 0.001) and Kiskdr (2.67 s; Z = 3.49, P < 0.001) individuals were significantly lower than that of Kisumu (3.8 s). The lifespan of the three ...
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Chemical composition and the insecticidal activity of Aeollanthus pubescens leaf essential oil against Anopheles gambiae sensu stricto
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Autor/in / Beteiligte Person: | Bohounton, Roméo Barnabé ; Djogbénou, Luc Salako ; Djihinto, Oswald Yédjinnavênan ; Dedome, Oronce Sedjro-Ludolphe ; Sovegnon, Pierre Marie ; Barea, Bruno ; Adomou, Aristide ; Villeneuve, Pierre ; Tchobo, Fidèle Paul ; Ecole Polytechnique d'Abomey Calavi (EPAC) ; Université d’Abomey-Calavi = University of Abomey Calavi (UAC) ; Liverpool School of Tropical Medicine (LSTM) ; Démarche intégrée pour l'obtention d'aliments de qualité (UMR QualiSud) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) ; Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) ; Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Wellcome Trust (intermediate fellowship in public health and tropical medicine no. 109917/Z/15/Z) |
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Zeitschrift: | ISSN: 1756-3305, 2021 |
Veröffentlichung: | HAL CCSD ; BioMed Central, 2021 |
Medientyp: | academicJournal |
DOI: | 10.1186/s13071-021-05012-w |
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