Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis
2024
Online
academicJournal
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Funding Information: This work has been funded by National funds, through the Foundation for Science and Technology (FCT)\u2013project UIDB/50026/2020 (DOI 10.54499/UIDB/50026/2020), UIDP/50026/2020 (DOI 10.54499/UIDP/50026/2020) and LA/P/0050/2020 (DOI 10.54499/LA/P/0050/2020); by ICVS Scientific Microscopy Platform, member of the national infrastructure PPBI\u2013Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122); and by the projects NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by the European Research Council (ERC) under the European Union\u2019s Horizon 2020 research and innovation programme (H2020-ERC-2017-STG-GA 759853-StemCellHabitat). Hugo Fernandes-Silva was supported by a doctoral fellowship (PD/BD/137655/2018) from FCT as part of the Inter-University Doctoral Programme in Ageing and Chronic Disease (PhDOC). Publisher Copyright: © 2024 by the authors. ; Lung branching morphogenesis relies on intricate epithelial–mesenchymal interactions and signaling networks. Still, the interplay between signaling and energy metabolism in shaping embryonic lung development remains unexplored. Retinoic acid (RA) signaling influences lung proximal–distal patterning and branching morphogenesis, but its role as a metabolic modulator is unknown. Hence, this study investigates how RA signaling affects the metabolic profile of lung branching. We performed ex vivo lung explant culture of embryonic chicken lungs treated with DMSO, 1 µM RA, or 10 µM BMS493. Extracellular metabolite consumption/production was evaluated by using 1H-NMR spectroscopy. Mitochondrial respiration and biogenesis were also analyzed. Proliferation was assessed using an EdU-based assay. The expression of crucial metabolic/signaling components was examined through Western blot, qPCR, and in situ hybridization. RA signaling ...
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Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis
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Autor/in / Beteiligte Person: | Fernandes-Silva, Hugo ; Alves, Marco G. ; Garcez, Marcia R. ; Correia-Pinto, Jorge ; Oliveira, Pedro F. ; Homem, Catarina C.F. ; Moura, Rute S. ; iNOVA4Health - pólo NMS ; NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) |
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Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1661-6596 (print) |
DOI: | 10.3390/ijms25095054 |
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