Hepatoprotective effect of pretreatment with rosemary and ginger essential oil in experimental model of acetaminophen-induced injury

The objective of this study went to investigate and evaluate the hepatic protection and antioxidant effects of the essential oil of ginger (GEO) and rosemary (REO), against hepatic damage in male mice of BALB/c induced by the experimental of liver injury acetaminophen-induced model. The experimental animals have been divided into eight groups with n=5. Each group received by gavage for seven days following treatment: Group (1) control group received no treatment. Group (2) received only vehicle saline containing 0.1% Tween 80, REO or GEO. Groups (3, 4 and 5) received pre-treatment with GEO at doses of 125, 250 and 500 mg/kg. Groups (6, 7 and 8) received pre-treated with REO at doses of 125, 250, and 500 mg/kg. On the seventh day after receiving pretreatment with oil, the animals were fasted for a period of eight hours. After this period animals of Group second to eighth acetaminophen received by gavage at the dose of 250 mg/kg. After 12 h, the mice were anesthetized with halothane, blood was collected to determine plasma markers alanine (ALT) and aspartate aminotransferase (AST) in serum. Sections of liver samples were used to determine the activity of the enzyme myeloperoxidase (MPO). Lipid peroxidation assays were performed in homogenates was used as egg yolk lipid rich medium. The oil GEO and REO were evaluated at concentrations of 0.5, 5.0, 50.0 and 500x10-3 mg/ml. Ascorbic acid was used as positive control. DPPH assays were made with both GEO and REO oils at concentrations from 3.21 to 100 mg/ml. Ascorbic acid was used as positive control. Data were expressed as mean ± SEM for each group. The results were statistically analyzed by analysis of variance (One-way ANOVA) followed by Tukey test. Differences were considered significant at p