Application of Alanine Scanning to Determination of Amino Acids Essential for Peptide Adsorption at the Solid/Solution Interface and Binding to the Receptor: Surface-Enhanced Raman/Infrared Spectroscopy versus Bioactivity Assays
In: Journal of Medicinal Chemistry, Jg. 64 (2021-06-10), S. 8410-8422
Online
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Zugriff:
The article describes the application of the alanine-scanning technique used in combination with Raman, surface-enhanced Raman, attenuated total reflection Fourier transform infrared, and surface-enhanced infrared absorption (SEIRA) spectroscopies, which allowed defining the role of individual amino acid residues in the C-terminal 6–14 fragment of the bombesin chain (BN6–14) on the path of its adsorption on the surface of Ag (AgNPs) and Au nanoparticles (AuNPs). A reliable analysis of the SEIRA spectra of these peptides was possible, thanks to a curve fitting of these spectra. By combining alanine-scanning with biological activity studies using cell lines overexpressing bombesin receptors and the intracellular inositol monophosphate assay, it was possible to determine which peptide side chains play a significant role in binding a peptide to membrane-bound G protein-coupled receptors (GPCRs). Based on the analysis of spectral profiles and bioactivity results, conclusions for the specific peptide–metal and peptide–GPCR interactions were drawn and compared.
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Application of Alanine Scanning to Determination of Amino Acids Essential for Peptide Adsorption at the Solid/Solution Interface and Binding to the Receptor: Surface-Enhanced Raman/Infrared Spectroscopy versus Bioactivity Assays
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Autor/in / Beteiligte Person: | Domin, Helena ; Makowska, Marta ; Burnat, Grzegorz ; Proniewicz, Edyta ; Prahl, Adam ; Małuch, Izabela |
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Zeitschrift: | Journal of Medicinal Chemistry, Jg. 64 (2021-06-10), S. 8410-8422 |
Veröffentlichung: | American Chemical Society (ACS), 2021 |
Medientyp: | unknown |
ISSN: | 1520-4804 (print) ; 0022-2623 (print) |
DOI: | 10.1021/acs.jmedchem.1c00397 |
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