Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter
In: Scientific Reports, Jg. 9 (2019), Heft 1, S. 1-10
Online
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Zugriff:
Class switch recombination (CSR), which targets exclusively the constant region of the immunoglobulin heavy chain (IgH) locus, plays an important role in humoral immunity by generating different antibody effector functions. The IgH constant locus contains multiple genes controlled by isotype (I) promoters induced by extracellular signals that activate specific I promoters, leading to B cell commitment. However, it is unknown whether after initial commitment to one promoter, non-responsive I promoters are irreversibly silent or if they can be activated after exposure to their specific inducers. Here, we studied the murine cell line CH12, which is committed to produce IgA in response to TGF-β. We show that, although other promoters than Iα are transcriptionally inactive, they are not irreversibly silent. Following deletion of the committed Iα promoter by CRISPR/Cas9, other I promoters display a complex transcriptional pattern largely dependent on the initial committing signal.
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Essential role of the initial activation signal in isotype selection upon deletion of a transcriptionally committed promoter
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Autor/in / Beteiligte Person: | Oudinet, Chloé ; Ahmed Amine Khamlichi ; Schöne, Lisa ; Dauba, Audrey ; Santos, Joana M. ; Institut de pharmacologie et de biologie structurale (IPBS) ; Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3) ; Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées ; CEntre de REcherches en MAthématiques de la DEcision (CEREMADE) ; Université Paris Dauphine-PSL ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) ; LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs) ; Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) ; Cellules Dendritiques, Immunomodulation et Greffes ; Université de Tours ; Université Toulouse III - Paul Sabatier (UT3) ; Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS) |
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Zeitschrift: | Scientific Reports, Jg. 9 (2019), Heft 1, S. 1-10 |
Veröffentlichung: | Nature Publishing Group, 2019 |
Medientyp: | unknown |
ISSN: | 2045-2322 (print) |
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