Initiator methionine tRNA is essential for Ty1 transposition in yeast
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 89 (1992-04-15), Heft 8
Online
unknown
Zugriff:
The yeast retrotransposon Ty1 transposes through an RNA intermediate by a mechanism similar to that of retroviral reverse transcription and integration. Ty1 RNA contains a putative minus strand primer binding site (-PBS) that is complementary to the 3' acceptor stem of the initiator methionine tRNA (tRNA(iMet)). Here we demonstrate that the tRNA(iMet) is used as a primer for Ty1 reverse transcription. Mutations in the Ty1 element that alter 5 of 10 nucleotides that are complementary to the tRNA(iMet) abolish Ty1 transposition, even though they are silent with regard to Ty1 protein coding. We have constructed a yeast strain lacking wild-type tRNA(iMet) that is dependent on a mutant derivative of tRNA(iMet) that has an altered acceptor stem sequence, engineered to restore homology with the Ty1 -PBS mutant. The compensatory mutations made in the tRNA(iMet) alleviate the transposition defect of the Ty1 -PBS mutant. The mutant and wild-type tRNA(iMet) are enriched within Ty1 virus-like particles irrespective of complementarity to the Ty1 -PBS. Thus, complementarity between the Ty1 -PBS and tRNA(iMet) is essential for transposition but is not necessary for packaging of the tRNA inside virus-like particles.
Titel: |
Initiator methionine tRNA is essential for Ty1 transposition in yeast
|
---|---|
Autor/in / Beteiligte Person: | Byström, Anders S. ; Chapman, Karen B. ; Boeke, Jef D. |
Link: | |
Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 89 (1992-04-15), Heft 8 |
Veröffentlichung: | 1992 |
Medientyp: | unknown |
ISSN: | 0027-8424 (print) |
Schlagwort: |
|
Sonstiges: |
|