Essential Amino Acid Supplementation by Gut Microbes of a Wood-Feeding Cerambycid
Oxford University Press, 2015
Online
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Zugriff:
Insects are unable to synthesize essential amino acids (EAAs) de novo, thus rely on dietary or symbiotic sources for them. Wood is a poor resource of nitrogen in general, and EAAs in particular. In this study, we investigated whether gut microbiota of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), a cerambycid that feeds in the heartwood of healthy host trees, serve as sources of EAAs to their host under different dietary conditions. δ 13 C-stable isotope analyses revealed significant δ 13 C-enrichment (3.4 ± 0.1‰; mean ± SEM) across five EAAs in wood-fed larvae relative to their woody diet. δ 13 C values for the consumers greater than 1‰ indicate significant contributions from non-dietary EAA sources (symbionts in this case). In contrast, δ 13 C-enrichment of artificial diet-fed larvae (controls) relative to their food source was markedly less (1.7 ± 0.1‰) than was observed in wood-fed larvae, yet still exceeded the threshold of 1‰. A predictive model based on δ 13 C EAA signatures of five EAAs from representative bacterial, fungal, and plant samples identified symbiotic bacteria and fungi as the likely supplementary sources of EAA in wood-fed larvae. Using the same model, but with an artificial diet as the dietary source, we identified minor supplementary bacterial sources of EAA in artificial diet-fed larvae. This study highlights how microbes associated with A. glabripennis can serve as a source of EAAs when fed on nutrient-limited diets, potentially circumventing the dietary limitations of feeding on woody substrates.
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Essential Amino Acid Supplementation by Gut Microbes of a Wood-Feeding Cerambycid
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Autor/in / Beteiligte Person: | Hoover, Kelli ; Rosa, Cristina ; Ayayee, Paul A. ; Ferry, James G. ; Felton, Gary W. ; Larsen, Thomas |
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Veröffentlichung: | Oxford University Press, 2015 |
Medientyp: | unknown |
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