TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail
In: Biochemical and Biophysical Research Communications, Jg. 565 (2021-08-01), S. 91-96
Online
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Zugriff:
Amphibians such as Xenopus tropicalis exhibit a remarkable capacity for tissue regeneration after traumatic injury. Although transforming growth factor-β (TGF-β) receptor signaling is known to be essential for tissue regeneration in fish and amphibians, the role of TGF-β ligands in this process is not well understood. Here, we show that inhibition of TGF-β1 function prevents tail regeneration in Xenopus tropicalis tadpoles. We found that expression of tgfb1 is present before tail amputation and is sustained throughout the regeneration process. CRISPR-mediated knock-out (KO) of tgfb1 retards tail regeneration; the phenotype of tgfb1 KO tadpoles can be rescued by injection of tgfb1 mRNA. Cell proliferation, a critical event for the success of tissue regeneration, is downregulated in tgfb1 KO tadpoles. In addition, tgfb1 KO reduces the expression of phosphorylated Smad2/3 (pSmad2/3) which is important for TGF-β signal-mediated cell proliferation. Collectively, our results show that TGF-β1 regulates cell proliferation through the activation of Smad2/3. We therefore propose that TGF-β1 plays a critical role in TGF-β receptor-dependent tadpole tail regeneration in Xenopus.
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TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail
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Autor/in / Beteiligte Person: | Moriyama, Yuka ; Nakamura, Makoto ; Suzuki, Atsushi ; Wlizla, Marcin ; Yoshida, Hitoshi ; Horb, Marko E. ; Takebayashi-Suzuki, Kimiko ; Kawakita, Itsuki |
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Zeitschrift: | Biochemical and Biophysical Research Communications, Jg. 565 (2021-08-01), S. 91-96 |
Veröffentlichung: | Elsevier BV, 2021 |
Medientyp: | unknown |
ISSN: | 0006-291X (print) |
DOI: | 10.1016/j.bbrc.2021.05.082 |
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