DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication
In: Journal of Cell Science, Jg. 102 (1992-07-01), S. 393-400
Online
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Zugriff:
The RCC1 protein that is required for coupling mitosis with the S phase has a DNA-binding domain in the N-terminal region outside the repeat. We found that RCC1 protein without any DNA-binding activity complemented the tsBN2 mutation with the same efficiency as that of intact RCC1 protein. In ts+ transformants of tsBN2 cells transfected with the RCC1 cDNA lacking the DNA-binding domain, an endogenous RCC1 disappeared at 39.5 degrees C, and the deleted RCC1 protein encoded by the transfected cDNA was found in the cytoplasm, but a significant amount of it was also found in the nuclei. This deleted RCC1 protein was eluted from the nuclei with the same concentration of NaCl and DNase I as was used for the intact RCC1 protein in BHK21 cells. Furthermore, the deleted RCC1 protein co-migrated with the nucleosome fraction on sucrose density gradient analysis. These results indicate that the RCC1 protein binds chromatin with the aid of other unknown protein(s). Thus, the DNA-binding domain of RCC1 protein is not essential for coupling between the S and M phases, but was shown instead to function as a nuclear translocation signal.
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DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication
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Autor/in / Beteiligte Person: | Seino, Hiroaki ; Hisamoto, Naoki ; Uzawa, Satoru ; Sekiguchi, Takeshi ; Nishimoto, Takeharu |
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Zeitschrift: | Journal of Cell Science, Jg. 102 (1992-07-01), S. 393-400 |
Veröffentlichung: | The Company of Biologists, 1992 |
Medientyp: | unknown |
ISSN: | 1477-9137 (print) ; 0021-9533 (print) |
DOI: | 10.1242/jcs.102.3.393 |
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