Interactive effects of supplemental ultraviolet-B radiation and indole-3-acetic acid on Coleus forskohlii Briq.: Alterations in morphological-, physiological-, and biochemical characteristics and essential oil content

Ultraviolet (UV)-B radiation and the growth hormone indole-3-acetic acid (IAA) have been known to cause various changes in plants at morphological and physiological levels as individual entities, but their interactive effects on the overall plant performance remain practically unknown. The present study was conducted under near-natural field conditions to evaluate the effects of supplemental (s)-UV-B (ambient+3.6kJm-2day-1) treatment alone, and in combination with two doses of IAA (200ppm and 400ppm) exogenously applied as foliar spray on various growth-, morphological-, physiological-, and biochemical parameters of an indigenous medicinal plant, Coleus forskohlii. Under s-UV-B, the plant growth and morphology were adversely affected (along with reductions in protein- and chlorophyll contents) with concomitant increase in secondary metabolites (as substantiated by an increase in the activities of various enzymes of the phenylpropanoid pathway) and cumulative antioxidative potential (CAP), suggesting the plant's capability of adaptive resilience against UV-B. The essential oil content of the plant was, however, compromised reducing its pharmaceutical value. IAA application at both doses led to a reversal in the effects caused by s-UV-B radiation alone; both the plant growth as well as the essential oil content improved, especially at the higher IAA dose, suggesting its ameliorative role against UV-B induced oxidative stress, and also in improving the plant's medicinal value.