EF-P Is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues
In: Science, Jg. 339 (2013-01-04), S. 85-88
Online
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Zugriff:
Translating Polyproline Translation of messenger RNA into protein is carried out by the ribosome, together with a variety of accessory factors, which offer the potential for regulation of this critical step in gene expression (see the Perspective by Buskirk and Green ). Ude et al. (p. 82 , published online 13 December), using bacterial genetics and an in vitro reconstituted translation system, and Doerfel et al. (p. 85 , published online 13 December), using a model assay for peptide bond formation, find that the universally conserved bacterial elongation factor P (EF-P) (which is orthologous to the archaeal and eukaryotic initiation factor 5A) is required for the efficient translation of polyproline-containing polypeptides. Such short polyproline stretches (with runs of two, three, or more proline residues) would otherwise cause ribosomal stalling.
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EF-P Is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues
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Autor/in / Beteiligte Person: | Doerfel, Lili K. ; Wohlgemuth, Ingo ; Urlaub, Henning ; Rodnina, Marina V. ; Kothe, Christina ; Peske, Frank |
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Zeitschrift: | Science, Jg. 339 (2013-01-04), S. 85-88 |
Veröffentlichung: | American Association for the Advancement of Science (AAAS), 2013 |
Medientyp: | unknown |
ISSN: | 1095-9203 (print) ; 0036-8075 (print) |
DOI: | 10.1126/science.1229017 |
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