CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
In: Journal of Cell Biology, Jg. 150 (2000-07-10), S. 1-12
Online
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Zugriff:
CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.
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CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
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Autor/in / Beteiligte Person: | Yucel, Jennifer K. ; Marszalek, Janet D. ; McIntosh, J. Richard ; Goldstein, Lawrence S.B. ; Cleveland, Don W. ; Philp, Alastair Valentine |
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Zeitschrift: | Journal of Cell Biology, Jg. 150 (2000-07-10), S. 1-12 |
Veröffentlichung: | Rockefeller University Press, 2000 |
Medientyp: | unknown |
ISSN: | 1540-8140 (print) ; 0021-9525 (print) |
DOI: | 10.1083/jcb.150.1.1a |
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