Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe
American Society for Biochemistry and Molecular Biology, 2017
Online
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Zugriff:
A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G1 phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome maintenance protein complex, origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element Δ9 of the Schizosaccharomyces pombe DNA-replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5′-(A/T)n(C/G)(A/T)9–10(G/C)(A/T)n-3′ (n ≥ 1). We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.
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Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe
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Autor/in / Beteiligte Person: | Hua, Yu ; Yang, Fang ; Zhang, Yuan ; Jin, Changwen ; Wang, Tao ; Hu, Jiazhi ; Kong, Daochun ; Zhang, Yi ; Ding, Jienv ; Hu, Yunfei ; He, Peng ; Guan, Ling ; Ye, Qiong |
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Veröffentlichung: | American Society for Biochemistry and Molecular Biology, 2017 |
Medientyp: | unknown |
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