Identification of ATPases Pontin and Reptin as Telomerase Components Essential for Holoenzyme Assembly
In: Cell, , Heft 6, S. 945-957
Online
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Zugriff:
Telomerase is a multi-subunit ribonucleoprotein (RNP) complex that adds telomere repeats to the ends of linear chromosomes. Three essential telomerase components have been identified thus far: the telomerase reverse transcriptase (TERT), the telomerase RNA component (TERC), and the TERC-binding protein dyskerin. Few other proteins are known to be required for human telomerase function, significantly limiting our understanding of both telomerase regulation and mechanisms of telomerase action. Here, we identify the ATPases pontin and reptin as telomerase components through affinity purification of TERT from human cells. Pontin interacts with both TERT and dyskerin, and the amount of TERT bound to pontin and reptin peaks in S phase, evidence for dynamic cell cycle-dependent regulation of TERT. Depletion of pontin and reptin markedly impairs accumulation of the telomerase RNP, indicating an essential role in telomerase assembly. These findings reveal an unanticipated requirement for additional enzymes in telomerase RNP biogenesis and suggest new approaches for inhibiting telomerase in human cancer.
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Identification of ATPases Pontin and Reptin as Telomerase Components Essential for Holoenzyme Assembly
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Autor/in / Beteiligte Person: | Mason, Philip J. ; Veenstra, Timothy D. ; Artandi, Steven E. ; Meng, Zhaojing ; Venteicher, Andrew S. |
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Zeitschrift: | Cell, , Heft 6, S. 945-957 |
Veröffentlichung: | Elsevier Inc. |
Medientyp: | unknown |
ISSN: | 0092-8674 (print) |
DOI: | 10.1016/j.cell.2008.01.019 |
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