Agarwood Essential Oil Displays Sedative-Hypnotic Effects through the GABAergic System
In: Molecules; Volume 22; Issue 12; Pages: 2190, Jg. 22 (2017-12-09), Heft 12, p 2190
Online
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Zugriff:
Although agarwood has been used as a tranquilizer in Asian countries for hundreds of years, the underlying pharmacological basis is still unclear. This study investigated the sedative-hypnotic effect of agarwood essential oil (AEO) using locomotor activity and pentobarbital-induced sleeping assays in mice. Single (1-day) and multiple (7- and 14-days) administrations of 60 mg/kg AEO generated significant sedative effect on inhibiting locomotor activity and hypnotic effect on pentobarbital-induced sleeping in mice. Interestingly, prolonged AEO treatment did not result in obvious desensitization. Concoitant measurement of the levels of brain neurotransmitters using ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) indicated that AEO had no significant effect on the levels of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in the brain. However, the sedative-hypnotic effects were blocked by the type A GABA (GABAA) receptor antagonists bicuculline and flumazenil. In addition, AEO significantly elevated the expression of GABAA receptor subunits and subtypes in the cerebral cortex. Furthermore, AEO increased chlorine ion (Cl-) influx through GABAA receptors in human neuroblastoma cells. These results together demonstrate that AEO exerts its sedative-hypnotic effects through regulating gene expression of GABAA receptors and potentiating GABAA receptor function.
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Agarwood Essential Oil Displays Sedative-Hypnotic Effects through the GABAergic System
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Autor/in / Beteiligte Person: | Wang, Shuai ; Liu, Xinmin ; Guo, Peng ; Peng, Deqian ; Canhong, Wang ; Wei, Jianhe ; Wu, Chongming |
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Zeitschrift: | Molecules; Volume 22; Issue 12; Pages: 2190, Jg. 22 (2017-12-09), Heft 12, p 2190 |
Veröffentlichung: | Multidisciplinary Digital Publishing Institute, 2017 |
Medientyp: | unknown |
ISSN: | 1420-3049 (print) |
DOI: | 10.3390/molecules22122190 |
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