Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis

Miyazaki, Jun-ichi ; Takemoto, Noriaki ; et al.

In: PLOS ONE, Jg. 11 (2016-03-01), Heft 3, p e0150390, S. e0150390

Online unknown

Zugriff:

The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.

Titel:	Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
Autor/in / Beteiligte Person:	Miyazaki, Jun-ichi ; Takemoto, Noriaki ; Yoshimura, Takuji ; Tashiro, Fumi ; Miyazaki, Satsuki
Link:	View record at OpenAIRE (Volltext) https://doi.org/10.1371/journal.pone.0150390
Zeitschrift:	PLOS ONE, Jg. 11 (2016-03-01), Heft 3, p e0150390, S. e0150390
Veröffentlichung:	Public Library of Science (PLoS), 2016
Medientyp:	unknown
ISSN:	1932-6203 (print)
DOI:	10.1371/journal.pone.0150390
Schlagwort:	Male 0301 basic medicine Physiology lcsh:Medicine Fluorescent Antibody Technique Artificial Gene Amplification and Extension Retrotransposon Biochemistry Polymerase Chain Reaction Reproductive Physiology Animal Cells Mobile Genetic Elements Testis Medicine and Health Sciences Testes Cell Cycle and Cell Division lcsh:Science Cells, Cultured Mice, Knockout Zinc finger Regulation of gene expression DNA methylation Multidisciplinary Chromosome Biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation, Developmental Genomics Complementary DNA Spermatids Chromatin Nucleic acids Meiosis Retrotransposons Cell Processes Argonaute Proteins Epigenetics Female Anatomy Cellular Types DNA modification Genital Anatomy Chromatin modification Research Article Protein Binding Mice, 129 Strain Protein family Forms of DNA Immunoblotting Molecular Sequence Data Biology Research and Analysis Methods Cell Line Zinc Finger Domain 03 medical and health sciences Genetic Elements Gonocyte Protein Domains Genetics Animals Amino Acid Sequence Spermatogenesis Molecular Biology Techniques Molecular Biology Gene Sequence Homology, Amino Acid lcsh:R Reproductive System Transposable Elements Biology and Life Sciences Proteins Cell Biology DNA Reverse Transcriptase-Polymerase Chain Reaction Molecular biology Sperm Mice, Inbred C57BL Germ Cells 030104 developmental biology lcsh:Q Gene expression Carrier Proteins
Sonstiges:	Nachgewiesen in: OpenAIRE Rights: OPEN

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