An Essential Role of N-Terminal Arginylation in Cardiovascular Development
In: Science, Jg. 297 (2002-07-05), S. 96-99
Online
unknown
Zugriff:
The enzymatic conjugation of arginine to the N-termini of proteins is a part of the ubiquitin-dependent N-end rule pathway of protein degradation. In mammals, three N-terminal residues—aspartate, glutamate, and cysteine—are substrates for arginylation. The mouse ATE1 gene encodes a family of Arg-tRNA-protein transferases (R-transferases) that mediate N-terminal arginylation. We constructed ATE1-lacking mouse strains and found that ATE1 −/− embryos die with defects in heart development and in angiogenic remodeling of the early vascular plexus. Through biochemical analyses, we show that N-terminal cysteine, in contrast to N-terminal aspartate and glutamate, is oxidized before its arginylation by R-transferase, suggesting that the arginylation branch of the N-end rule pathway functions as an oxygen sensor.
Titel: |
An Essential Role of N-Terminal Arginylation in Cardiovascular Development
|
---|---|
Autor/in / Beteiligte Person: | Hu, Ronggui ; Jai Wha Seo ; Varshavsky, Alexander ; Davydov, Ilia V. ; Du, Fangyong ; Jee Young An ; Kashina, Anna ; Yong Tae Kwon |
Link: | |
Zeitschrift: | Science, Jg. 297 (2002-07-05), S. 96-99 |
Veröffentlichung: | American Association for the Advancement of Science (AAAS), 2002 |
Medientyp: | unknown |
ISSN: | 1095-9203 (print) ; 0036-8075 (print) |
DOI: | 10.1126/science.1069531 |
Schlagwort: |
|
Sonstiges: |
|