A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells
In: Nature Communications, Jg. 7 (2016), Heft 1, S. 1-14
Online
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Zugriff:
Mammalian chromosome replication starts from distinct sites; however, the principles governing initiation site selection are unclear because proteins essential for DNA replication do not exhibit sequence-specific DNA binding. Here we identify a replication-initiation determinant (RepID) protein that binds a subset of replication-initiation sites. A large fraction of RepID-binding sites share a common G-rich motif and exhibit elevated replication initiation. RepID is required for initiation of DNA replication from RepID-bound replication origins, including the origin at the human beta-globin (HBB) locus. At HBB, RepID is involved in an interaction between the replication origin (Rep-P) and the locus control region. RepID-depleted murine embryonic fibroblasts exhibit abnormal replication fork progression and fewer replication-initiation events. These observations are consistent with a model, suggesting that RepID facilitates replication initiation at a distinct group of human replication origins.
Origins of mammalian DNA replication are poorly characterised because they lack an Identifiable consensus sequence. Here the authors identify RepID, a protein that binds to a subset of G-rich replication origins and facilitates initiation from those origins.
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A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells
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Autor/in / Beteiligte Person: | Aladjem, Mirit I. ; Reinhold, William C. ; Fu, Haiqing ; You, Yang ; Hanna, Harlington ; Boisclair, Yves R. ; Huang, Liang ; Rao, Mishal ; Ryan, Michael T. ; Redon, Christophe E. ; Smith, Owen K. ; Utani, Koichi ; Chii Mei Lin ; Kim, RyangGuk ; Long, Qiaoming ; Zhang, Ya |
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Zeitschrift: | Nature Communications, Jg. 7 (2016), Heft 1, S. 1-14 |
Veröffentlichung: | Nature Portfolio, 2016 |
Medientyp: | unknown |
ISSN: | 2041-1723 (print) |
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