A conserved RNA structure is essential for a satellite RNA-mediated inhibition of helper virus accumulation
In: Nucleic Acids Research, Jg. 47 (2019-07-03), S. 8255-8271
Online
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Zugriff:
As a class of parasitic, non-coding RNAs, satellite RNAs (satRNAs) have to compete with their helper virus for limited amounts of viral and/or host resources for efficient replication, by which they usually reduce viral accumulation and symptom expression. Here, we report a cucumber mosaic virus (CMV)-associated satRNA (sat-T1) that ameliorated CMV-induced symptoms, accompanied with a significant reduction in the accumulation of viral genomic RNAs 1 and 2, which encode components of the viral replicase. Intrans replication assays suggest that the reduced accumulation is the outcome of replication competition. The structural basis of sat-T1 responsible for the inhibition of viral RNA accumulation was determined to be a three-way branched secondary structure that contains two biologically important hairpins. One is indispensable for the helper virus inhibition, and the other engages in formation of a tertiary pseudoknot structure that is essential for sat-T1 survival. The secondary structure containing the pseudoknot is the first RNA element with a biological phenotype experimentally identified in CMV satRNAs, and it is structurally conserved in most CMV satRNAs. Thus, this may be a generic method for CMV satRNAs to inhibit the accumulation of the helper virus via the newly-identified RNA structure.
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A conserved RNA structure is essential for a satellite RNA-mediated inhibition of helper virus accumulation
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Autor/in / Beteiligte Person: | Du, Zhiyou ; Liao, Qiansheng ; Wang, Qian ; Gu, Zhouhang ; Palukaitis, Peter ; He, Lu |
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Zeitschrift: | Nucleic Acids Research, Jg. 47 (2019-07-03), S. 8255-8271 |
Veröffentlichung: | Oxford University Press (OUP), 2019 |
Medientyp: | unknown |
ISSN: | 1362-4962 (print) ; 0305-1048 (print) |
DOI: | 10.1093/nar/gkz564 |
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