Global Regulation of H2A.Z Localization by the INO80 Chromatin-Remodeling Enzyme Is Essential for Genome Integrity
In: Cell, Jg. 144 (2011), Heft 2, S. 200-213
Online
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Zugriff:
INO80 is an evolutionarily conserved, ATP-dependent chromatin remodeling enzyme that plays roles in transcription, DNA repair, and replication. Here, we show that yeast INO80 facilitates these diverse processes at least in part by controlling genome-wide distribution of the histone variant H2A.Z. In the absence of INO80, H2A.Z nucleosomes are mis-localized, and H2A.Z levels at promoters show reduced responsiveness to transcriptional changes, suggesting that INO80 controls H2A.Z dynamics. Additionally, we demonstrate that INO80 has a novel histone exchange activity in which the enzyme can replace nucleosomal H2A.Z/H2B with free H2A/H2B dimers. Genetic interactions between ino80 and htz1 support a model in which INO80 catalyzes the removal of unacetylated H2A.Z from chromatin as a novel mechanism to promote genome stability.
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Global Regulation of H2A.Z Localization by the INO80 Chromatin-Remodeling Enzyme Is Essential for Genome Integrity
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Autor/in / Beteiligte Person: | Peterson, Craig L. ; Papamichos-Chronakis, Manolis ; Rando, Oliver J. ; Watanabe, Shinya |
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Zeitschrift: | Cell, Jg. 144 (2011), Heft 2, S. 200-213 |
Veröffentlichung: | Elsevier BV, 2011 |
Medientyp: | unknown |
ISSN: | 0092-8674 (print) |
DOI: | 10.1016/j.cell.2010.12.021 |
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