Molecular crowding creates an essential environment for the formation of stable G-quadruplexes in long double-stranded DNA
In: Nucleic Acids Research, Jg. 38 (2009-10-01), Heft 1, S. 327-338
Online
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Zugriff:
Large numbers of guanine-rich sequences with potential to form G-quadruplexes have been identified in genomes of various organisms. Such sequences are constrained at both ends by long DNA duplex with a complementary strand in close proximity to compete for duplex formation. G-quadruplex/duplex competition in long double-stranded DNA has rarely been studied. In this work, we used DMS footprinting and gel electrophoresis to study G-quadruplex formation in long double-stranded DNA derived from human genome under both dilute and molecular crowding condition created by PEG. G-quadruplex formation was observed in the process of RNA transcription and after heat denaturation/renaturation under molecular crowding condition. Our results showed that the heat denaturation/renaturation treatment followed by gel electrophoresis could provide a simple method to quantitatively access the ability of G-quadruplex formation in long double-stranded DNA. The effect of K(+) and PEG concentration was investigated and we found that stable G-quadruplexes could only form under the crowding condition with PEG at concentrations near the physiological concentration of biomass in living cells. This observation reveals a physical basis for the formation of stable G-quadruplexes in genome and supports its presence under the in vivo molecular crowding condition.
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Molecular crowding creates an essential environment for the formation of stable G-quadruplexes in long double-stranded DNA
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Autor/in / Beteiligte Person: | Chen, Zhao ; Hao, Yu-hua ; Zheng, Ke-wei ; Tan, Zheng |
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Zeitschrift: | Nucleic Acids Research, Jg. 38 (2009-10-01), Heft 1, S. 327-338 |
Veröffentlichung: | Oxford University Press, 2009 |
Medientyp: | unknown |
ISSN: | 1362-4962 (print) ; 0305-1048 (print) |
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