Cortical Organization by the Septin Cytoskeleton Is Essential for Structural and Mechanical Integrity of Mammalian Spermatozoa
In: Developmental Cell, , Heft 3, S. 343-352
Online
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Zugriff:
Summary Septins are polymerizing GTP binding proteins required for cortical organization during cytokinesis and other cellular processes. A mammalian septin gene Sept4 is expressed mainly in postmitotic neural cells and postmeiotic male germ cells. In mouse and human spermatozoa, SEPT4 and other septins are found in the annulus, a cortical ring which separates the middle and principal pieces. Sept4 −/− male mice are sterile due to defective morphology and motility of the sperm flagellum. In Sept4 null spermatozoa, the annulus is replaced by a fragile segment lacking cortical material, beneath which kinesin-mediated intraflagellar transport stalls. The sterility is rescued by injection of sperm into oocytes, demonstrating that each Sept4 null spermatozoon carries an intact haploid genome. The annulus/septin ring is also disorganized in spermatozoa from a subset of human patients with asthenospermia syndrome. Thus, cortical organization based on circular assembly of the septin cytoskeleton is essential for the structural and mechanical integrity of mammalian spermatozoa.
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Cortical Organization by the Septin Cytoskeleton Is Essential for Structural and Mechanical Integrity of Mammalian Spermatozoa
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Autor/in / Beteiligte Person: | Goto, Motohito ; Kinoshita, Ayae ; Tanaka, Hiromitsu ; Yamada, Shuichi ; Itohara, Shigeyoshi ; Kinoshita, Makoto ; Ogawa, Osamu ; Takahashi, Chiaki ; Noda, Makoto ; Tanigaki, Ai ; Okubo, Kazutoshi ; Ihara, Masafumi ; Nishimune, Yoshitake ; Kitano, Ayumi ; Nishiyama, Hiroyuki |
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Zeitschrift: | Developmental Cell, , Heft 3, S. 343-352 |
Veröffentlichung: | Elsevier Inc. |
Medientyp: | unknown |
ISSN: | 1534-5807 (print) |
DOI: | 10.1016/j.devcel.2004.12.005 |
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