Casein synthesis is independently and additively related to individual essential amino acid supply
In: Journal of Dairy Science, Jg. 97 (2014-05-01), S. 2998-3005
Online
unknown
Zugriff:
Specific AA affect rates of milk protein synthesis in the mammary glands of lactating cows. The objective of this study was to quantify the rate of αS1-casein synthesis in response to Ile, Leu, Met, and Thr supplementation, and to test the single-limiting AA theory for milk protein synthesis by exploring interactions among these AA. Effects of Ile, Leu, Met, and Thr were studied in vitro with a composite design containing a central point repeated 4 times, with 2 axial points per AA and a complete 2(4) factorial. Other AA were at the concentration in Dulbecco's modified Eagle medium/F12 medium (DMEM). The experiment was replicated with mammary tissue from 5 lactating cows. Mammary tissue slices (0.12 ± 0.02 g) were incubated for 4h at 37°C in 5 mL of treatment medium containing (2)H5-Phe. Caseins were precipitated from cell homogenate supernatants. Enrichment with (2)H5-Phe of the N[34]LLRFFVAPFPE αS1 peptide was determined by matrix-assisted laser desorption/ionization-tandem time-of-flight (MALDI-TOF-TOF), which was used to determine enrichment of Phe in the transfer (t)RNA pool and αS1-casein fractional synthesis rates (CFSR). Data were analyzed with a polynomial mixed model containing linear, quadratic, and 2-factor interactions for Ile, Leu, Met, and Thr, and cow and residual as random factors. Interactions were not significant at P
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Casein synthesis is independently and additively related to individual essential amino acid supply
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Autor/in / Beteiligte Person: | Hanigan, Mark D. ; Lin, X.Y. ; Singer, L.M. ; Helm, Richard F. ; McGilliard, M.L. ; S.I. Arriola Apelo ; St-Pierre, Normand R. ; Ray, William K. |
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Zeitschrift: | Journal of Dairy Science, Jg. 97 (2014-05-01), S. 2998-3005 |
Veröffentlichung: | American Dairy Science Association, 2014 |
Medientyp: | unknown |
ISSN: | 0022-0302 (print) |
DOI: | 10.3168/jds.2013-7422 |
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