Fa1p is a 171 kDa protein essential for axonemal microtubule severing in Chlamydomonas
In: Journal of Cell Science, Jg. 113 (2000-06-01), S. 1963-1971
Online
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Zugriff:
A key event in deflagellation or deciliation is the severing of the nine outer-doublet axonemal microtubules at a specific site in the flagellar transition zone. Previous genetic analysis revealed three genes that are essential for deflagellation in Chlamydomonas. We have now identified the first of these products, Fa1p, a protein required for Ca(2+)-dependent, axonemal microtubule severing. Genetic mapping and the availability of a tagged allele allowed us to physically map the gene to the centromere-proximal domain of the mating-type locus. We identified clones of Chlamydomonas genomic DNA that rescued the Ca(2+)-dependent axonemal microtubule severing defect of fa1 mutants. The FA1 cDNA, obtained by RT-PCR, encodes a novel protein of 171 kDa, which is predicted to contain an amino-terminal coiled-coil domain and three Ca(2+)/calmodulin binding domains. By western analysis and subcellular fractionation, the FA1 product is enriched in flagellar-basal body complexes. Based on these observations and previous studies, we hypothesize that a Ca(2+)-activated, Ca(2+)-binding protein binds Fa1p leading ultimately to the activation of axonemal microtubule severing.
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Fa1p is a 171 kDa protein essential for axonemal microtubule severing in Chlamydomonas
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Autor/in / Beteiligte Person: | Griffis, Eric R. ; Quarmby, Lynne M. ; Kim, Peter J. ; Finst, Rip J. |
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Zeitschrift: | Journal of Cell Science, Jg. 113 (2000-06-01), S. 1963-1971 |
Veröffentlichung: | The Company of Biologists, 2000 |
Medientyp: | unknown |
ISSN: | 1477-9137 (print) ; 0021-9533 (print) |
DOI: | 10.1242/jcs.113.11.1963 |
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