A Rare SNP Identified a TCP Transcription Factor Essential for Tendril Development in Cucumber
In: Molecular Plant, Jg. 8 (2015-12-01), Heft 12, S. 1795-1808
Online
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Zugriff:
Rare genetic variants are abundant in genomes but less tractable in genome-wide association study. Here we exploit a strategy of rare variation mapping to discover a gene essential for tendril development in cucumber (Cucumis sativus L.). In a collection of >3000 lines, we discovered a unique tendril-less line that forms branches instead of tendrils and, therefore, loses its climbing ability. We hypothesized that this unusual phenotype was caused by a rare variation and subsequently identified the causative single nucleotide polymorphism. The affected gene TEN encodes a TCP transcription factor conserved within the cucurbits and is expressed specifically in tendrils, representing a new organ identity gene. The variation occurs within a protein motif unique to the cucurbits and impairs its function as a transcriptional activator. Analyses of transcriptomes from near-isogenic lines identified downstream genes required for the tendril's capability to sense and climb a support. This study provides an example to explore rare functional variants in plant genomes.
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A Rare SNP Identified a TCP Transcription Factor Essential for Tendril Development in Cucumber
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Autor/in / Beteiligte Person: | Lin, Xingzhong ; Yang, Qing ; Wang, Shenhao ; Tian, Nana ; Huang, Sanwen ; Lin, Tao ; Xu, Mengnan ; Zhang, Zhonghua ; Yang, Xueyong ; Qi, Jianjian ; Shao, Guangjin |
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Zeitschrift: | Molecular Plant, Jg. 8 (2015-12-01), Heft 12, S. 1795-1808 |
Veröffentlichung: | Elsevier BV, 2015 |
Medientyp: | unknown |
ISSN: | 1674-2052 (print) |
DOI: | 10.1016/j.molp.2015.10.005 |
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