MalF is essential for persistence of Mycoplasma gallisepticum in vivo
In: Microbiology, Jg. 159 (2013-07-01), S. 1459-1470
Online
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Zugriff:
International audience; There is limited understanding of the molecular basis of virulence in the important avian pathogen Mycoplasma gallisepticum. To define genes that may be involved in colonization of chickens, a collection of mutants of the virulent Ap3AS strain of M. gallisepticum were generated by signature-tagged transposon mutagenesis. The collection included mutants with single insertions in the genes encoding the adhesin GapA and the cytadherence-related protein CrmA, and Western blotting confirmed that these mutants did not express these proteins. In two separate in vivo screenings, two GapA-deficient mutants (ST mutants 02-1 and 06-1) were occasionally recovered from birds, suggesting that GapA expression may not always be essential for persistence of strain Ap3AS. CrmA-deficient ST mutant 33-1 colonized birds poorly and had reduced virulence, indicating that CrmA was a significant virulence factor, but was not absolutely essential for colonization. ST mutant 04-1 contained a single transposon insertion in malF, a predicted ABC sugar transport permease, and could not be reisolated even when inoculated by itself into a group of birds, suggesting that expression of MalF was essential for persistence of M. galliseptium strain Ap3AS in infected birds.
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MalF is essential for persistence of Mycoplasma gallisepticum in vivo
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Autor/in / Beteiligte Person: | Rosengarten, Renate ; Browning, Glenn F. ; Kanci, Anna ; Tseng, Chi-Wen ; Chen, Zheng-Hong ; Geary, Steven J. ; Markham, Philip F. ; Citti, Christine ; Chiu, Chien-Ju ; University of Melbourne ; Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées ; Institute of Bacteriology, Mycology and Hygiene ; University of Veterinary Medicine [Vienna] (Vetmeduni) ; Guiyang Medical University ; University of Connecticut (UCONN) ; Australian Egg Corporation Ltd ; Australian Poultry, CRC |
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Zeitschrift: | Microbiology, Jg. 159 (2013-07-01), S. 1459-1470 |
Veröffentlichung: | Microbiology Society, 2013 |
Medientyp: | unknown |
ISSN: | 1465-2080 (print) ; 1350-0872 (print) |
DOI: | 10.1099/mic.0.067553-0 |
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