Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis
In: Developmental cell, Jg. 15 (2008-03-24), Heft 2, S. 285-97
Online
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Contains fulltext : 70649.pdf (Publisher’s version ) (Closed access) Tight control of transposon activity is essential for the integrity of the germline. Recently, a germ-cell-specific organelle, nuage, was proposed to play a role in transposon repression. To test this hypothesis, we disrupted a murine homolog of a Drosophila nuage protein Maelstrom. Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs) were observed. In the adult Mael(-/-) testes, LINE-1 (L1) derepression occurred at the onset of meiosis. As a result, Mael(-/-) spermatocytes were flooded with L1 ribonucleoproteins (RNPs) that accumulated in large cytoplasmic enclaves and nuclei. Mael(-/-) spermatocytes with nuclear L1 RNPs exhibited massive DNA damage and severe chromosome asynapsis even in the absence of SPO11-generated meiotic double-strand breaks. This study demonstrates that MAEL, a nuage component, is indispensable for the silencing of TEs and identifies the initiation of meiosis as an important step in TE control in the male germline.
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Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis
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Autor/in / Beteiligte Person: | Martin, Sandra L. ; Goodheart, Mary L. ; Boer, Peter ; Godfried W. van der Heijden ; Hardiman, Tara C. ; Bortvin, Alex ; Sarah F.C. Soper |
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Zeitschrift: | Developmental cell, Jg. 15 (2008-03-24), Heft 2, S. 285-97 |
Veröffentlichung: | 2008 |
Medientyp: | unknown |
ISSN: | 1878-1551 (print) ; 1534-5807 (print) |
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