Both structural and non-structural forms of the readthrough protein of cucurbit aphid-borne yellows virus are essential for efficient systemic infection of plants
In: PLoS ONE, Jg. 9 (2014), Heft 4
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Zugriff:
International audience; Cucurbit aphid-borne yellows virus (CABYV) is a polerovirus (Luteoviridae family) with a capsid composed of the major coat protein and a minor component referred to as the readthrough protein (RT). Two forms of the RT were reported: a full-length protein of 74 kDa detected in infected plants and a truncated form of 55 kDa (RT*) incorporated into virions. Both forms were detected in CABYV-infected plants. To clarify the specific roles of each protein in the viral cycle, we generated by deletion a polerovirus mutant able to synthesize only the RT* which is incorporated into the particle. This mutant was unable to move systemically from inoculated leaves inferring that the C-terminal half of the RT is required for efficient long-distance transport of CABYV. Among a collection of CABYV mutants bearing point mutations in the central domain of the RT, we obtained a mutant impaired in the correct processing of the RT which does not produce the RT*. This mutant accumulated very poorly in upper non-inoculated leaves, suggesting that the RT* has a functional role in long-distance movement of CABYV. Taken together, these results infer that both RT proteins are required for an efficient CABYV movement.
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Both structural and non-structural forms of the readthrough protein of cucurbit aphid-borne yellows virus are essential for efficient systemic infection of plants
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Autor/in / Beteiligte Person: | Erdinger, Monique ; Ziegler-Graff, Véronique ; Brault, Véronique ; Boissinot, Sylvaine ; Monsion, Baptiste ; Santé de la vigne et qualité du vin (SVQV) ; Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg, I ; Unité Propre de Recherche 2357 ; Institution de Biologie Moléculaire des Plantes ; Institut de biologie moléculaire des plantes (IBMP) ; Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
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Zeitschrift: | PLoS ONE, Jg. 9 (2014), Heft 4 |
Veröffentlichung: | Public Library of Science (PLoS), 2014 |
Medientyp: | unknown |
ISSN: | 1932-6203 (print) |
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