Regulation of Starch Stores by a Ca2+-Dependent Protein Kinase Is Essential for Viable Cyst Development in Toxoplasma gondii
In: Cell Host & Microbe, Jg. 18 (2015-12-01), S. 670-681
Online
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Zugriff:
Transmissible stages of Toxoplasma gondii store energy in the form of the carbohydrate amylopectin. Here, we show that the Ca(2+)-dependent protein kinase CDPK2 is a critical regulator of amylopectin metabolism. Increased synthesis and loss of degradation of amylopectin in CDPK2 deficient parasites results in the hyperaccumulation of this sugar polymer. A carbohydrate-binding module 20 (CBM20) targets CDPK2 to amylopectin stores, while the EF-hands regulate CDPK2 kinase activity in response to Ca(2+) to modulate amylopectin levels. We identify enzymes involved in amylopectin turnover whose phosphorylation is dependent on CDPK2 activity. Strikingly, accumulation of massive amylopectin granules in CDPK2-deficient bradyzoite stages leads to gross morphological defects and complete ablation of cyst formation in a mouse model. Together these data show that Ca(2+) signaling regulates carbohydrate metabolism in Toxoplasma and that the post-translational control of this pathway is required for normal cyst development.
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Regulation of Starch Stores by a Ca2+-Dependent Protein Kinase Is Essential for Viable Cyst Development in Toxoplasma gondii
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Autor/in / Beteiligte Person: | Bacic, Antony ; Dagley, Laura F. ; Blume, Martin ; Tonkin, Christopher J. ; Uboldi, Alessandro D. ; Gooley, Paul R. ; Masters, Seth L. ; Beahan, Cherie T. ; McCoy, James M. ; Gerlic, Motti ; Webb, Andrew I. ; Ferguson, David J. P. ; McConville, Malcolm J. ; Stapleton, David |
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Zeitschrift: | Cell Host & Microbe, Jg. 18 (2015-12-01), S. 670-681 |
Veröffentlichung: | Elsevier BV, 2015 |
Medientyp: | unknown |
ISSN: | 1931-3128 (print) |
DOI: | 10.1016/j.chom.2015.11.004 |
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