Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis

Che, Mi-Hyun ; Ye, Mi-Kyung ; et al.

In: Molecules, Jg. 25 (2020-10-02), Heft 4517, p 4517, S. 4517-4517

Online unknown

Zugriff:

The present study aims to investigate the immunomodulatory effects of essential oil from Chamaecyparis obtusa (EOCO) in an ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model. BALB/c mice were intraperitoneally sensitized and stimulated with OVA. From day 22 to 35, 0.01% and 0.1% ECOC was intranasally administered 1 h before OVA stimulation. Nasal symptoms, as well as serum total and OVA-specific immunoglobulin (Ig) E levels, were measured. Interleukin (IL)-4, IL-10, interferon (IFN)-&gamma

and tumor necrosis factor (TNF)-&alpha

levels in nasal lavage fluid (NLF) and their production by activated splenocytes were measured. Histological changes in the sinonasal mucosa were evaluated through hematoxylin and eosin and periodic acid-Schiff staining procedure. Th cytokines and their transcription factor mRNA expressions were determined using reverse-transcription polymerase chain reaction. Intranasal EOCO administration significantly suppressed allergic symptoms, OVA-specific IgE level, sinonasal mucosal inflammatory cell infiltration, and mucus-producing periodic acid-Schiff (PAS) positive cell count. EOCO also significantly inhibited IL-4, IL-10, and TNF-&alpha

levels in NLF and activated splenocytes. Th2 and Treg related cytokines and their transcription factors in sinonasal mucosa were significantly suppressed through intransal EOCO instillation. In conclusion, repetitive EOCO intranasal instillation showed anti-inflammatory and anti-allergic effects by suppressing nasal symptoms and inhibiting the production and expression of inflammatory mediators in the OVA-induced AR mouse model.

Titel:	Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis
Autor/in / Beteiligte Person:	Che, Mi-Hyun ; Ye, Mi-Kyung ; Shin, Seung-Heon ; Lee, Dong-Won
Link:	View record at OpenAIRE (Volltext) https://doi.org/10.3390/molecules25194517
Zeitschrift:	Molecules, Jg. 25 (2020-10-02), Heft 4517, p 4517, S. 4517-4517
Veröffentlichung:	MDPI AG, 2020
Medientyp:	unknown
ISSN:	1420-3049 (print)
DOI:	10.3390/molecules25194517
Schlagwort:	medicine.medical_treatment H&E stain Pharmaceutical Science Immunoglobulin E Analytical Chemistry 0302 clinical medicine Drug Discovery cytokine transcription factor Mice, Inbred BALB C 0303 health sciences biology Interleukin respiratory system Cytokine Chemistry (miscellaneous) 030220 oncology & carcinogenesis Chamaecyparis obtusa Molecular Medicine Female Tumor necrosis factor alpha Nasal Lavage Fluid Inflammation Mediators Ovalbumin Article essential oil lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Oils, Volatile medicine Animals Immunologic Factors Physical and Theoretical Chemistry 030304 developmental biology allergic rhinitis business.industry Organic Chemistry Rhinitis, Allergic Disease Models, Animal Nasal Mucosa Chamaecyparis Immunology biology.protein Nasal administration business Spleen Transcription Factors
Sonstiges:	Nachgewiesen in: OpenAIRE File Description: application/pdf Rights: OPEN

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