The cadherin-like protein is essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecticidal CryIAa toxin
In: FEBS Letters, , Heft 2, S. 385-390
Online
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Zugriff:
The Bacillus thuringiensis CryIAa toxin binds a cadherin-like protein (BtR175) on the brush-border membranes of the Bombyx mori midgut columnar cells, which are the targets. By introducing the BtR175 gene with a baculovirus, Spodoptera frugiperda Sf9 cells expressed BtR175 protein on the cell membrane and became susceptible to the CryIAa toxin. The toxin bound the cadherin repeat adjacent to the membrane and made a pore that passed inorganic ions, causing the cell to swell and burst. This was not observed with a BtR175 variant lacking the toxin-binding site. This in vitro experiment mimicked the specific insecticidal action of the toxin in vivo well.
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The cadherin-like protein is essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecticidal CryIAa toxin
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Autor/in / Beteiligte Person: | Nagamatsu, Yasunori ; Sasaki, Kazuhiro ; Furukawa, Yasuo ; Koike, Takashi ; Yoshimoto, Akihiro |
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Zeitschrift: | FEBS Letters, , Heft 2, S. 385-390 |
Veröffentlichung: | Federation of European Biochemical Societies. Published by Elsevier B.V. |
Medientyp: | unknown |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/S0014-5793(99)01327-7 |
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