Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival
In: Journal of immunology (Baltimore, Md. : 1950), Jg. 180 (2008-06-05), Heft 12
Online
unknown
Zugriff:
Tec family kinases have important roles in lymphocytes; however, little is known about their function in monocytes/macrophages. In this study we report that Tec family kinases are essential for M-CSF (M-CSF)-induced signaling pathways that regulate macrophage survival. Compared with wild-type bone marrow-derived macrophage (BMM) cultures, Tec−/−Btk−/− BMM cultures displayed increased cell death that correlated with a severe drop in macrophage numbers. In addition, macrophages deficient in either Tec or Btk showed expression and activation of caspase-11. Elucidation of M-CSF receptor (M-CSFR) signaling pathways revealed that the total tyrosine phosphorylation pattern upon M-CSF stimulation was altered in Tec−/−Btk−/− macrophages despite normal expression and phosphorylation of the M-CSFR. Further, Tec and Btk are required for proper expression of the GM-CSF receptor α (GM-CSFRα) chain in macrophages but not dendritic cells, implicating Tec family kinases in the lineage-specific regulation of GM-CSFRα expression. Taken together, our study shows that Tec and Btk regulate M-CSFR signaling-induced macrophage survival and provides a novel link between Tec family kinases and the regulation of caspase-11 and GM-CSFRα expression.
Titel: |
Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival
|
---|---|
Autor/in / Beteiligte Person: | Unger, Bernd ; Ellmeier, Wilfried ; Alitalo, Kari ; Schmidt, Uwe ; Melcher, Martin ; Rajantie, Iiro |
Link: | |
Zeitschrift: | Journal of immunology (Baltimore, Md. : 1950), Jg. 180 (2008-06-05), Heft 12 |
Veröffentlichung: | 2008 |
Medientyp: | unknown |
ISSN: | 0022-1767 (print) |
Schlagwort: |
|
Sonstiges: |
|