The Rhizobium etli trpB Gene Is Essential for an Effective Symbiotic Interaction with Phaseolus vulgaris
In: Molecular Plant-Microbe Interactions®, Jg. 12 (1999-10-01), S. 926-933
Online
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Zugriff:
A mutant strain (CTNUX4) of Rhizobium etli carrying Tn5 unable to grow with ammonium as the sole nitrogen source was isolated and characterized. Sequence analysis showed that Tn5 is inserted into a trpB (tryptophan synthase)-homologous gene. When tested on the roots of Phaseolus vulgaris, strain CTNUX4 was able to induce only small, slightly pink, ineffective (Fix¯) nodules. However, under free-living conditions, strain CTNUX4 was unable to produce flavonoid-inducible lipo-chitin oligosaccharides (Nod factors) unless tryptophan was added to the growth medium. These data and histological observations indicate that the lack of tryptophan biosynthesis affects the symbiotic behavior of R. etli.
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The Rhizobium etli trpB Gene Is Essential for an Effective Symbiotic Interaction with Phaseolus vulgaris
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| Autor/in / Beteiligte Person: | Cermola, Michele ; Riccio, Anna ; Patriarca, Eduardo J. ; Caputo, Emilia ; Tatè, Rosarita ; Favre, Renée |
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| Zeitschrift: | Molecular Plant-Microbe Interactions®, Jg. 12 (1999-10-01), S. 926-933 |
| Veröffentlichung: | Scientific Societies, 1999 |
| Medientyp: | unknown |
| ISSN: | 1943-7706 (print) ; 0894-0282 (print) |
| DOI: | 10.1094/mpmi.1999.12.10.926 |
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