Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function
In: Nature Communications, Jg. 9 (2018), Heft 1, S. 1-14
Online
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Zugriff:
Plasmacytoid dendritic cells (pDC) are essential for immune competence. Here we show that pDC precursor differentiated from human CD34+ hematopoietic stem and progenitor cells (HSPC) has low surface expression of pDC markers, and has limited induction of type I interferon (IFN) and IL-6 upon TLR7 and TLR9 agonists treatment; by contrast, cGAS or RIG-I agonists-mediated activation is not altered. Importantly, after priming with type I and II IFN, these precursor pDCs attain a phenotype and functional activity similar to that of peripheral blood-derived pDCs. Data from CRISPR/Cas9-mediated genome editing of HSPCs further show that HSPC-pDCs with genetic modifications can be obtained, and that expression of the IFN-α receptor is essential for the optimal function, but dispensable for the differentiation, of HSPC-pDC percursor. Our results thus demonstrate the biological effects of IFNs for regulating pDC function, and provide the means of generating of gene-modified human pDCs.
Plasmacytoid dendritic cells (pDC) are an important regulator of immune responses. Here the authors show that pDC precursors, similar to peripheral blood-derived pDCs, can be differentiated from human CD34+ hematopoietic stem and progenitor cells, with type I/II IFN priming being required for their functional maturation and differentiation.
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Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function
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Autor/in / Beteiligte Person: | Porteus, Matt ; Uldbjerg, Niels ; Roan, Nadia R. ; Kjær, L ; Pillai, Satish K. ; Laustsen, Anders ; J H Egedahl ; Denton, Paul W. ; Krapp, Christian ; Hai Q Tang ; Jakobsen, Martin R. ; Bak, Rasmus O. ; Charlotte Christie Petersen ; Nyegaard, Mette |
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Zeitschrift: | Nature Communications, Jg. 9 (2018), Heft 1, S. 1-14 |
Veröffentlichung: | Nature Publishing Group, 2018 |
Medientyp: | unknown |
ISSN: | 2041-1723 (print) |
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