The cyclic AMP response element plays an essential role in the expression of the human prostaglandin-endoperoxide synthase 2 gene in differentiated U937 monocytic cells
In: FEBS Letters, Jg. 350 (1994-08-15), S. 51-54
Online
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Zugriff:
The promoter activity of 1432 bp upstream of the human prostaglandin-endoperoxide synthase 2 gene (PTGS2) was examined in differentiated U937 monocytic cells expressing prostaglandin-endoperoxide synthase 2 mRNA. Transient transfection experiments were performed using these cells and reporter vectors containing the upstream region of the gene with deletions or site-specific mutations and the luciferase gene. The deletion or destruction of the cyclic AMP response element (nucleotides −59 to −53) markedly reduced the promoter activity of this gene. Electrophoretic mobility shift assays showed that a nuclear protein(s) binding to the cyclic AMP response element was induced during monocytic differentiation of U937 cells. These results indicate that expression of the human prostaglandin-endoperoxide synthase 2 gene in differentiated U937 monocytic cells is regulated by the cyclic AMP response element.
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The cyclic AMP response element plays an essential role in the expression of the human prostaglandin-endoperoxide synthase 2 gene in differentiated U937 monocytic cells
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Autor/in / Beteiligte Person: | Hara, Shuntaro ; Nanayama, Toyomichi ; Tanabe, Tadashi ; Inoue, Hiroyasu ; Yokoyama, Chieko |
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Zeitschrift: | FEBS Letters, Jg. 350 (1994-08-15), S. 51-54 |
Veröffentlichung: | Wiley, 1994 |
Medientyp: | unknown |
ISSN: | 0014-5793 (print) |
DOI: | 10.1016/0014-5793(94)00731-4 |
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